The software allows differential protein expression analysis of conventional 2-DE and 2D-DIGE gels, to detect protein abundance changes for biomarker discovery. (Fig.1). SWISS-2DPAGE Proteins on 2-D and SDS PAGE maps Proteins & Proteomes Software tool Myristoylator N-terminal myristoylation of proteins by neural networks. SWISS-2DPAGE is an annotated database that assembles data on proteins from a variety of human and mouse biological samples as well as from Arabidopsis thaliana, Dictyostelium discoideum, Escherichia coli, Saccharomyces cerevisiae and Staphylococcus aureus.In all cases, proteins have been identified on 2-D polyacrylamide gel electrophoresis (PAGE) reference maps. and SWISS-2DPAGE can also be queried using SRS. It allows supplementary ways to browse the database, in particular: ExPASy (1,2) is the main host for the following databases that are partially or completely developed at the SIB in Geneva: 1. What you can do with this resource? Description The HUPO Proteomics Standards Initiative ... SWISS-2DPAGE reference map (analytical gel) Description protocol (analytical gel) for SWISS-2DPAGE reference map Gel documents The new system has been built with the Make2D-DB II, a tool (freely available from ExPASy) to create, convert, interconnect and keep up-to-date proteomics databases. SWISS-3DIMAGE is an image database which strives to provide high quality pictures of biological macromolecules with known three-dimensional structure.The database contains mostly images of experimentally elucidated structures, but also provides views of well accepted theoretical protein models. The SWISS-PROT knowledgebase (3,4) (http://www.expasy.org/sprot/) is a curated protein sequence database, which strives to provide high quality annotations (such as the description of the function of a protein, its domain structure, post-translational modifications and variants), a minimal level of redundancy and a high level of integration with other databases. SWISS-2DPAGE is an annotated two-dimensional polyacrylamide gel electro-phoresis (2-DE) database. If you have submitted private content and need to recover it, please contact the ExPASy helpdesk. The April-2005 release contains over 4000 spots from 1265 entries in 36 reference maps from human, mouse, Arabidopsis thaliana, Dictyostelium discoideum, Escherichia coli, Saccharomyces cerevisiae, and Staphylococcus aureus (N315). Melanie combines a unique and flexible interface for the visualization, exploration and analysis of 2D gel and blot images. Several scientific categories: ExPASy is the SIB Bioinformatics Resource Portal which provides access to scientific databases and software tools in different areas of life sciences including proteomics, genomics, phylogeny, systems biology, evolution, population genetics, transcriptomics etc. gel electrophoresis in high-quality scientific databases and software tools using Expasy, the Swiss Bioinformatics Resource Portal. The original flat file format of all ExPASy databases is based on different line types, where a two-letter line code defines the The protein entries in SWISS-2DPAGE are text files structured to be readable by human as well as by computer programs. ExPASy databases include SWISS-PROT and TrEMBL, SWISS-2DPAGE, PROSITE, ENZYME and the SWISS-MODEL repository. Each entry is composed of defined lines, used to record various kinds of data (Fig. It contains manually-annotated (reviewed) records with information extracted from the literature and curator-evaluated computational analysis. FORMAT. No operations found for this resource. genes and genomes in high-quality scientific databases and software tools using Expasy, the Swiss Bioinformatics Resource Portal Compute pI/Mw for Swiss-Prot/TrEMBL entries or a user-entered sequence Please enter one or more UniProtKB/Swiss-Prot protein identifiers (ID) (e.g. SWISS-PROT is supplemented … Browse these keywords in Expasy Protein ... SWISS-2DPAGE Proteins on 2-D and SDS PAGE maps Proteins & Proteomes Software tool PeptideMass Theoretical protein cleavage by a given enzyme Proteins & Proteomes Software tool Myristoylator N-terminal myristoylation of … 2D PAGE maps for identified proteins : How to interpret a protein map; You may obtain an estimated location of the protein on various 2D PAGE maps, provided the whole amino acid sequence is known. The estimation is … The ExPASy (Expert Protein Analysis System) proteomics server of the Swiss Institute of Bioinformatics (SIB) is dedicated to the analysis of protein sequences and structures as well as 2-D PAGE (Disclaimer / References / Linking to ExPASy). UniProtKB/Swiss-Prot • protein sequence database • UniProtKB/Swiss-Prot • UniProtKB/Swiss-Prot is the manually annotated component of UniProtKB (produced by the UniProt consortium). The purpose of this server is to make protein modelling accessible to all life science researchers worldwide. 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